— The cooled sCMOS camera pairs a back-illuminated 4.2-megapixel sensor with model-specific low-noise options and acquisition up to 291 fps in a specified mode.
Weak-light experiments require a camera to collect scarce photons without giving up image detail or timing. The Revealer Gloria 6504 is a cooled scientific sCMOS camera built around Gpixel’s back-illuminated GSENSE6504BSI sensor.
The sensor records 2,048 by 2,048 pixels, or about 4.2 megapixels, with a 6.5-micrometer pixel pitch. Gpixel lists peak quantum efficiency of 95 percent at 450 nanometers and average quantum efficiency above 50 percent from 200 to 300 nanometers. Back illumination reduces obstruction from front-side wiring; for a given photon flux, the high quantum efficiency can yield more detected photoelectrons. Actual response depends on the emission wavelength and optical path.
Manufacturer-provided Single-molecule fluorescence signals captured with the Revealer Gloria 6504 sCMOS camera.
Read noise matters when the signal is small. Gpixel reports sensor-level read noise of about 0.43 electrons rms in low-noise mode at 100 frames per second. At camera level, published typical read noise is about 0.7 electrons for Gloria 6504 and 0.4 electrons for Gloria 6504 Pro. These figures describe different models and measurement conditions. Low read noise can help distinguish faint signals, but the camera is an integrating sCMOS device, not a SPAD detector that counts individual photons.
The camera also offers high full-frame acquisition rates. Its manufacturer lists up to 291 fps at 2,048 by 2,048 in 12-bit high-speed, high-sensitivity mode through CoaXPress, and up to 170 fps in 16-bit HDR mode. The rates apply to different readout modes. The combination of 4.2-megapixel resolution and high frame rate can preserve spatial detail while increasing temporal sampling, with mode choice based on signal level and experiment timing.
For longer exposures, thermoelectric cooling helps control thermal background. Gpixel lists dark current of about 0.004 electrons per pixel per second for the sensor at minus 30 degrees Celsius; product documentation lists exposures from about 11 microseconds to 30 seconds. The sensor figure does not establish camera dark current at every setting, so users should check background with dark frames at the actual exposure and cooling conditions.
The Gloria 6504’s value lies in this combination of high photon collection, low-noise model options, full-frame speed and cooling. It is relevant to weak-fluorescence microscopy and other experiments that need spatial detail and time-resolved imaging; actual performance depends on wavelength, optics, operating mode and sample conditions.
Contact Info:
Name: Harrison Shawn
Email: Send Email
Organization: HF Agile Device Co., Ltd.
Website: http://www.revealerhighspeed.com
Release ID: 89206574
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